Cytotoxicity and Genotoxicity of Metal Oxide Nanoparticles in Human Pluripotent Stem Cell-Derived Fibroblasts

Advances in the use of nanoparticles (NPs) has created promising progress biotechnology and consumer-care based industry. This an increasing need for testing their safety toxicity profiles. Hence, efforts to understand cellular responses towards nanomaterials are needed. However, current methods using animal cancer-derived cell lines raise questions on physiological relevance. In this aspect, study, we investigated pluripotent human embryonic stem cell- (hESCs) derived fibroblasts (hESC-Fib) as a closer representative vivo response well encourage 3Rs (replacement, reduction refinement) concept evaluating cytotoxic genotoxic effects zinc oxide (ZnO), titanium dioxide (TiO2) silicon-dioxide (SiO2) NPs. Cytotoxicity assays demonstrated that adverse respective NPs were observed hESC-Fib beyond concentrations 200 µg/mL (SiO2 NPs), 30 (TiO2 NPs) 20 (ZnO NPs). Flow cytometry results correlated with increased apoptosis upon increase NP concentration. Subsequently, scratch wound showed ZnO (10 µg/mL) TiO2 (20 inhibit rate coverage. DNA damage confirmed genotoxic. summary, could be used alternative platform profiles metal